Journal: Frontiers in Cellular Neuroscience

Article Title: Human Mesenchymal Stem Cells Prevent Neurological Complications of Radiotherapy

doi: 10.3389/fncel.2019.00204

Figure Lengend Snippet: hMSC administration does not compromise survival of glioma-bearing mice. (A) Schematic representation of the study design. U87 glioma cells were intracranially transplanted into the striatum of nude mice. After 10 days, mice received whole-brain radiation (total dose of 10 Gy) and the day after, 5 × 10 5 hMSCs were administered intranasally once per week for 4 weeks and time of death was monitored. (B) Xenolight DiR-labeled U87 glioma cells were locally transplanted into the striatum of nude mice. Images show bioluminescence activity in a representative animal 24 h after cell transplantation. (C) Animal weight was measured during the course of the experiment. Note the weight loss after radiation exposure. (D) Kaplan–Meier curve showing the percentage of survival of glioma-bearing mice. Note that IR and IR+MSC mice exhibited similar response, increasing survival as compared to NonIR mice. (E) Histological images of brain tumors at the time of death (indicated as days post tumor implantation, PTI) in the last individual surviving for each experimental group. H&E: Hematoxylin and eosin staining. Scale bar 1 mm (25 μm for details). Data are represented as mean ± SEM. n = 11–12 per group. ∗ p < 0.0001 compared to U87 NonIR mice; Multiple t -test (C) , Log-rank test (D) .

Article Snippet: For evaluation of cell biodistribution, cultured hMSCs were incubated with 400 μg/mL XenoLight DiR fluorescent dye (Perkin Elmer, Inc., Boston, MA) for 30 min at 37°C before transplantation.

Techniques: Labeling, Activity Assay, Transplantation Assay, Tumor Implantation, Staining

Journal: Nature Communications

Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry

doi: 10.1038/s41467-024-49412-9

Figure Lengend Snippet: a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP DNA segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green fluorescent protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.

Article Snippet: A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China).

Techniques: Irradiation, Recombinant

Journal: Nature Communications

Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry

doi: 10.1038/s41467-024-49412-9

Figure Lengend Snippet: a Schematic illustration of two parC -eGFP DNAs being pushed through ParM polymerization in a giant unilamellar vesicles (GUV) and subsequent GUV division under laser irradiation. b Confocal microscopy images of GUV division ( b1 – b3 ) and enhanced green fluorescent protein (eGFP) expression at 37 °C ( b4 – b7 ). b1 – b3 indicate GUV deformation, filament splitting, and division into two daughter cells, respectively. The white arrows in b1 and b2 indicate parC -eGFP DNA. The ParM filament was split by laser irradiation (561 nm, 0.7 mW, 5 s). The scale bars are 10 μm. n = 3 independent replicates. c Schematic illustration of eGFP expression in two daughter cells. The corresponding fluorescence intensity (FI) in daughter GUV 1 ( d ) and daughter GUV 2 ( e ) as a function of time. The normalized fluorescence intensity of eGFP was calculated from three independent samples. The data were presented as the mean values ± SDs; n = 3 independent replicates. Source data are provided as a Source Data file.

Article Snippet: A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China).

Techniques: Irradiation, Confocal Microscopy, Expressing, Fluorescence

Journal: Life

Article Title: Santin (5,7-Dihydroxy-3,6,4′-Trimetoxy-Flavone) Enhances TRAIL-Mediated Apoptosis in Colon Cancer Cells

doi: 10.3390/life13020592

Figure Lengend Snippet: The effects of TRAIL combined with santin on the mitochondrial membrane potential (ΔΨm) in colon cancer cells. SW480 and SW620 cells were subject to incubation for 48 h with rhsTRAIL (concentration of 25–100 ng/mL) and/or with santin (25–100 μM). The fluorescent microscopic analysis of DePsipher staining was used to assess the ΔΨm loss in cancer cells (*** p < 0.001 compared with control, +++ p < 0.001 compared with santin, ### p < 0.001 compared with TRAIL).

Article Snippet: The mitochondrial membrane potentials were measured by The DePsipher Kit (R and D Systems, Minneapolis, MN) in fluorescence microscopy.

Techniques: Membrane, Incubation, Concentration Assay, Staining, Control